BUB1 Rabbit Polyclonal Antibody

CAT#: TA306432

Rabbit Polyclonal Bub1 Antibody

Product Images

Western blot analysis of bub1 in A-20 lysate with bub1 antibody at (A) 2 and (B) 4 ug/mL.

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  Immunocytochemistry of Bub1 in A20 cells with Bub1 antibody at 10 ug/mL.

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  Immunofluorescence of Bub1 in A20 cells with Bub1 antibody at 20 ug/mL.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: WB: 2 - 4 ug/mL, ICC: 10 ug/mL, IF: 20 ug/mL
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Bub1 antibody was raised against a 19 amino acid peptide from near the amino terminus of human Bub1.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Affinity chromatography purified via peptide column
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: BUB1 mitotic checkpoint serine/threonine kinase
• Database Link:
  NP_004327
  Entrez Gene 12235 MouseEntrez Gene 699 Human
  UniProt ID O43683
• Background: Bub1 was initially identified as the mammalian homolog to the yeast protein through a cDNA screen. Bub1 encodes a kinase involved in the formation of the spindle checkpoint, an important mechanism that ensures high fidelity mitotic chromosome segregation. It is thought to be required for assembly of a functional inner centromere, sister chromatid cohesion via targeting of the Shugoshim protein and metaphase congression. Bub1 functions by phosphorylating cdc20, a member of the mitotic checkpoint complex and activating the spindle checkpoint. A related protein kinase Bub3 interacts with Bub1 and targets it to kinetochores prior to chromosome alignment. Mutations in bub1 have been associated with aneuploidy and several forms of cancer.
• Synonyms: BUB1A; BUB1L; hBUB1

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation