LIS1 (PAFAH1B1) Rabbit Polyclonal Antibody

CAT#: TA306455

Rabbit Polyclonal LIS1 Antibody

Product Images

Western blot analysis of LIS1 in HeLa cell lysate with LIS1 antibody at (A) 0.5 and (B) 1 ug/mL.

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  Immunocytochemistry of LIS1 in Jurkat cells with LIS1 antibody at 2.5 ug/mL.

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  Immunofluorescence of LIS1 in Jurkat cells with LIS1 antibody at 20 ug/mL.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: WB: 0.5 - 1 ug/mL, IF: 20 ug/mL
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: LIS1 antibody was raised against a 14 amino acid peptide from near the carboxy terminus of human LIS1.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1ug/ul
• Purification: Affinity chromatography purified via peptide column
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: platelet activating factor acetylhydrolase 1b regulatory subunit 1
• Database Link:
  NP_000421
  Entrez Gene 18472 MouseEntrez Gene 83572 RatEntrez Gene 5048 Human
  UniProt ID P43034
• Background: Lissencephaly is a severe brain developmental disease characterized by the mislocalization of cortical neurons, a smooth cerebral surface, mental retardation, and seizures. Classical lissencephaly is caused by sporadic mutations in the LIS1 gene. While LIS1 is known to act in a pathway deactivating the lipid messenger platelet-activating factor, LIS1 forms a complex with Nudel and 14-3-3e which is then transported from neuronal cell bodies through the actions of DISC1 and KIF5A, a microtubule-dependent directed motor protein kinesin. Decreased expression of LIS1 blocked neural stem cell division, morphogenesis, and motility, suggesting that LIS1 plays an important role in neuronal cell proliferation and localization in the developing brain. At least two isoforms of LIS1 are known to exist.
• Synonyms: LIS1; LIS2; MDCR; MDS; NudF; PAFAH

Reference Data

• Protein Pathways: Ether lipid metabolism, Metabolic pathways