IRGM Rabbit Polyclonal Antibody

CAT#: TA306552

Rabbit Polyclonal IRGM Antibody

Product Images

Western blot analysis of IRGM expression in SK-N-SH cell lysate with IRGM antibody at (A) 1 and (B) 2ug/ml.

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  Immunohistochemistry of CD4 in human thymus tissue with CD4 antibody at 5ug/ml.

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  Immunofluorescence of IRGM in Human Brain tissue with IRGM antibody at 20ug/ml.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1 - 2 ug/mL
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: IRGM antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of the human IRGM. The immunogen is located within amino acids 30 - 80 of IRGM.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1ug/ul
• Purification: Affinity chromatography purified via peptide column
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: immunity-related GTPase M
• Database Link:
  NP_001139277
  Entrez Gene 303090 RatEntrez Gene 345611 Human
  UniProt ID A1A4Y4
• Background: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. Two of the strongest hits implicate genes IRGM and ATG16L1, which encode proteins thought to be critical to the autophagy pathway and being significantly associated with Crohn’s disease. In mouse, IRGM belongs to a family of gamma-interferon-induced GTP-binding proteins of approximately 48 kDa. Murine IRGM induces autophagy and generates large autolysosomal organelles as a mechanism for the elimination of intracellular Mycobacterium tuberculosis. Human IRGM is also involved in autophagy and plays a role in the control of intracellular pathogens and in the reduction of intracellular bacillary load.
• Synonyms: IFI1; IRGM1; LRG-47; LRG47