GPAM Rabbit Polyclonal Antibody

CAT#: TA306579

Rabbit Polyclonal GPAT1 Antibody

Product Images

Western blot analysis of GPAT1 in rat brain tissue lysate with GPAT1 antibody at (A) 1 and (B) 2ug/ml.

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  Immunohistochemistry of GPAT1 in mouse brain tissue with GPAT1 antibody at 5ug/ml.

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  Immunohistochemistry of GPAT1 in rat brain with GPAT1 antibody at 2.5ug/ml.

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  Immunofluorescence of GPAT1 in Rat Brain tissue with GPAT1 antibody at 20ug/ml.

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  Immunofluorescence of GPAT1 in mouse brain tissue with GPAT1 antibody at 20ug/ml.

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Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1 - 2 ug/mL, ICC: 2.5 ug/mL, IF: 20 ug/mL
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: GPAT1 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of the human GPAT1. The immunogen is located within amino acids 730 - 780 of GPAT1.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1ug/ul
• Purification: Affinity chromatography purified via peptide column
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: glycerol-3-phosphate acyltransferase, mitochondrial
• Database Link:
  NP_065969
  Entrez Gene 14732 MouseEntrez Gene 29653 RatEntrez Gene 57678 Human
  UniProt ID Q9HCL2
• Background: Glycerol-3-phosphate acyltransferase 1 (GPAT1), one of four known GPAT isoforms, is located on the mitochondrial outer membrane, allowing reciprocal regulation with carnitine palmitoyltransferase-1. It is thought to be critical for the development of hepatic steatosis; steatosis triggered by GPAT1 overexpression leads to hepatic and possibly peripheral insulin resistance. GPAT1 is transcriptionally upregulated by insulin and sterol regulatory element binding protein (SREBP-1) and downregulated by AMP-activated protein kinase. Mice deficient in GPAT1 exhibit decreased triacylglycerol (TAG) in cardiomyocytes even in high-fat diets, suggesting that GPAT1 contributes significantly to TAG accumulation in heart tissue during lipogenic or high fat diets. At least two isoforms of GPAT1 are known to exist.
• Synonyms: GPAT; GPAT1

Reference Data

• Protein Pathways: Glycerolipid metabolism, Glycerophospholipid metabolism, Metabolic pathways