SATB1 Rabbit Polyclonal Antibody

CAT#: TA306588

Rabbit Polyclonal SATB1 Antibody

Product Images

Western blot analysis of SATB1 in A20 cell lysate with SATB1 antibody at (A) 2 and (B) 4 ug/ml. Below: Immunohistochemistry of SATB1 in human brain with SATB1 antibody at 5 ug/ml.

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  Immunohistochemistry of SATB1 in human brain with SATB1 antibody at 5 ug/ml.

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  Immunofluorescence of SATB1 in Human Brain cells with SATB1 antibody at 20 ug/mL.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 2 - 4 ug/mL, ICC: 5 ug/mL, IF: 20 ug/mL
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: SATB1 antibody was raised against a 15 amino acid peptide near the carboxy terminus of the human SATB1.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1ug/ul
• Purification: Affinity chromatography purified via peptide column
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: SATB homeobox 1
• Database Link:
  NP_001124482
  Entrez Gene 20230 MouseEntrez Gene 316164 RatEntrez Gene 6304 Human
  UniProt ID Q01826
• Background: Human special AT-rich sequence-binding protein-1 (SATB1) is a nuclear matrix/scaffold-associated region DNA-binding protein, predominantly expressed in the thymus and pre-B cells. Like its homolog SATB2, SATB1 selectively binds double-stranded, special AT-rich DNA sequences in which one strand exclusively consists of well-mixed A, T, and C nucleotides. SATB1 contains a dimerization domain that shares similarity with the PDZ motif, identified as an indispensable element for high-affinity binding of SATB1 to DNA. SATB1 constitutes a functional nuclear architecture that has a 'cage-like' protein distribution surrounding heterochromatin and regulates gene expression through chromatin remodeling/HDAC (histone deacetylase complex) and transcription factors recruitment. SATB1 functions as a 'genome organizer' essential for proper T-cell development. Recent studies show that SATB1 is necessary for breast cancer cells to become metastatic, and when ectopically expressed in non-metastatic cells, can induce invasive activity in vivo. At least two isoforms of SATB1 are known to exist.
• Synonyms: DNA-binding protein SATB1; SATB homeobox 1; scaffold-associating DNA's); special AT-rich sequence binding protein 1; special AT-rich sequence binding protein 1 (binds to nuclear matrix

Reference Data

• Protein Families: Druggable Genome, Transcription Factors