MED28 Rabbit Polyclonal Antibody

CAT#: TA306619

Rabbit Polyclonal MED28 Antibody

Product Images

Western blot analysis of MED28 in human brain tissue lysate with MED28 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.

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  Immunohistochemistry of MED28 in human brain tissue with MED28 antibody at 2.5 ug/ml.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 1 ug/mL, ICC: 2.5 ug/mL, IF: 20 ug/mL
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: MED28 antibody was raised against a 17 amino acid peptide from near the center of human MED28.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1ug/ul
• Purification: Affinity chromatography purified via peptide column
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: mediator complex subunit 28
• Database Link:
  NP_079481
  Entrez Gene 66999 MouseEntrez Gene 80306 Human
  UniProt ID Q9H204
• Background: The mediator complex is a multi-protein transcriptional co-activator that is expressed ubiquitously in eukaryotes from yeast to mammals and is required for induction of RNA polymerase II (pol II) transcription by DNA binding transcription factor. One of the proteins in this complex is MED28, also known as Magicin. MED28 is expressed in many cell lines and tissues. It has been shown that a down-regulation of MED28 expression in NIH3T3 cells results in a significant induction of several genes associated with smooth muscle cell differentiation and conversely its over-expression represses expression of SMC genes. MED28 can also form a ternary complex with Grb2 and Merlin, the neurofibromatosis 2 tumor suppressor protein, indicating that MED28 may play a role in Merlin’s tumor suppressive activity. MED28 has also been recently identified as an HIV dependency factor (HDF), suggesting that MED28 may be an important drug target in HIV treatment. At least two isoforms of MED28 are known to exist.
• Synonyms: 1500003D12Rik; EG1; magicin

Reference Data

• Protein Families: Transcription Factors