ASAH1 Rabbit Polyclonal Antibody

CAT#: TA306621

Rabbit Polyclonal ASAH1 Antibody

Product Images

Western blot analysis of ASAH1 in human heart tissue lysate with ASAH1 antibody at 1ug/ml.

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  Immunohistochemistry of ASAH1 in human heart tissue with ASAH1 antibody at 2.5ug/ml.

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  Immunofluorescence of ASAH1 in Human Heart cells with ASAH1 antibody at 20ug/ml.

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  Immunohistochemistry of ASAH1 in rat heart tissue with ASAH1 antibody at 5ug/ml.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1 - 2 ug/mL, ICC: 2.5 ug/mL, IF: 20 ug/mL
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: ASAH1 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of the human ASAH1. The immunogen is located within amino acids 240 - 290 of ASAH1.
• Formulation: PBS containing 0.02% sodium azide.
• Purification: Affinity chromatography purified via peptide column
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: N-acylsphingosine amidohydrolase (acid ceramidase) 1
• Database Link:
  NP_001120977
  Entrez Gene 11886 MouseEntrez Gene 84431 RatEntrez Gene 427 Human
  UniProt ID Q13510
• Background: Sphingolipids are hydrolyzed by ceramidases to yield sphingosine and fatty acids. These ceramidases are classified according to the pH range that supports their optimal activity. ASAH1 is an acid ceramidase and key regulator of ceramide metabolism. Mutations in this gene results in Farber Lipogranulomatosis, a fatal human genetic disorder that results in the painful swelling of the joints and tendons and pulminary insufficiency, while a complete knockout of its expression is lethal in mice. Recent studies have shown elevated levels of ASAH1 in Alzheimer’s disease (AD) patients correlating with a reduction in sphingomyelin and elevation of ceramide. Pretreatment of cultured neurons with recombinant AHAH1 prevented the cells from undergoing A-beta (Ab)-induced apoptosis. Multiple isoforms of this protein are known to exist.
• Synonyms: AC; ACDase; ASAH; PHP; PHP32; SMAPME

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Lysosome, Metabolic pathways, Sphingolipid metabolism