EDG1 (S1PR1) Rabbit Polyclonal Antibody

CAT#: TA306649

Rabbit Polyclonal S1P1 Antibody

Product Images

Western blot analysis of S1P1 in mouse thymus lysate with S1P1 antibody at (A) 1 and (B) 2ug/ml.

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  Immunohistochemistry of S1P1 in mouse thymus tissue with S1P1 antibody at 2.5ug/ml.

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  Immunohistochemistry of S1P1 in mouse thymus tissue with S1P1 antibody at 5ug/ml.

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  Immunofluorescence of S1P1 in mouse thymus tissue with S1P1 antibody at 20ug/ml.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1 - 2 ug/mL, ICC: 5 ug/mL
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: S1P1 antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of the human S1P1. The immunogen is located within the last 50 amino acids of S1P1.
• Formulation: PBS containing 0.02% sodium azide.
• Purification: Affinity chromatography purified via peptide column
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: sphingosine-1-phosphate receptor 1
• Database Link:
  NP_001391
  Entrez Gene 13609 MouseEntrez Gene 29733 RatEntrez Gene 1901 Human
  UniProt ID P21453
• Background: Movement of lymphocytes through lymphoid organs is required for generating immunity. Their migration into lymph nodes follows a series of events including integrin activation through chemokine signaling, adhesion and diapedis. The release of lymphocytes from lymph nodes is regulated by the phospholipid sphingosine-1-phosphate (S1P). One of its receptors S1P1 binds S1P with high specificity and affinity; agonism of this receptor by the immunosuppressive agent FTY720 inhibits the entry of tissue T cells into afferent lymphatics in homeostatic and inflammatory conditions. Recent experiments have indicated that CCR7-deficient T cells left lymph nodes more rapidly than wild-type cells did and these cells where also less effectively retained after treatment with FTY720, and that egress competence could be restored by inactivating G alpha i-protein-coupled receptor signaling. These results suggest that S1P1 acts in the lymphocyte to promote lymph node egress by overcoming retention signals mediated by CCR7 and G alpha i-protein-coupled receptor signaling. At least two isoforms of S1P1 are known to exist; this S1P1 antibody will only recognize the shorter isoform.
• Synonyms: CD363; CHEDG1; D1S3362; ECGF1; EDG-1; EDG1; S1P1

Reference Data

• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction