AAK1 Rabbit Polyclonal Antibody

CAT#: TA306661

Rabbit Polyclonal Aak1 Antibody

Product Images

Western blot analysis of Aak1 in A-20 lysate with Aak1 antibody at (A) 1 and (B) 2 ug/mL.

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  Immunocytochemistry of Aak1 in A-20 cells with Aak1 antibody at 5 ug/mL.

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  Immunofluorescence of aak1 in human brain tissue with aak1 antibody at 20 ug/mL.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: WB: 1 - 2 ug/mL, ICC: 5 ug/mL, IF: 20 ug/mL
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Aak1 antibody was raised against a 20 amino acid peptide near the amino terminus of the human Aak1.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1ug/ul
• Purification: Affinity chromatography purified via peptide column
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: AP2 associated kinase 1
• Database Link:
  NP_055726
  Entrez Gene 269774 MouseEntrez Gene 500244 RatEntrez Gene 22848 Human
  UniProt ID Q2M2I8
• Background: AP2-associated protein kinase 1 (Aak1) is a member of the Ark1/Prk1 subfamily of Ser/Thr protein kinases that are thought to regulate endocytosis by phosphorylating the accessory endocytic components. Aak1 interacts with and phosphorylates the mu2 subunit of the AP-2 complex, which promotes binding of the AP-2 to tyrosine based (Yxxf) internalization motif-containing receptors and subsequent receptor endocytosis. At least two isoforms of Aak1 are known to exist; the longer isoform contains an extended carboxy-terminus that contains an additional clathrin-binding domain. Overexpression of this long isoform or Aak1 depletion by RNA interference impairs transferrin recycling from the early/sorting endosome, suggesting that Aak1 functions at multiple steps of the endosomal pathway by regulating transferrin internalization and its recycling back to the plasma membrane.
• Synonyms: RP11-427H3.3

Reference Data

• Protein Families: Druggable Genome, Protein Kinase