MARCH8 Rabbit Polyclonal Antibody

CAT#: TA306677

Rabbit Polyclonal MARCH8 Antibody

Product Images

Western blot analysis of MARCH8 in HeLa cell lysate with MARCH8 antibody at (A) 0.5 ug/ml and (B) 1 ug/ml.

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  Immunocytochemistry of MARCH8 in HeLa cells with MARCH8 antibody at 2.5 ug/ml.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 0.5 - 1 ug/mL
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: MARCH8 antibody was raised against a 17 amino acid peptide from near the carboxy terminus of human MARCH8.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1ug/ul
• Purification: Affinity chromatography purified via peptide column
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: membrane associated ring-CH-type finger 8
• Database Link:
  NP_001002265
  Entrez Gene 71779 MouseEntrez Gene 312656 RatEntrez Gene 220972 Human
  UniProt ID Q5T0T0
• Background: MARCH8 (c-MIR) is a novel E3 ubiquitin ligase designated as the modulator of immune recognition (MIR) family, whose catalytic domain is a variant RING domain (RING-CH domain). MARCH8 was found as a functional and structural homolog of KSHV MIR1 and MIR2. MARCH8 targets B7-2 to lysosomal degradation and down-regulates the B7-2 surface expression through ubiquitination of its cytoplasmic tail. Furthermore, MARCH8 has been shown to down-regulate the expression of transferrin receptor and Fas, an important molecule for the induction of apoptosis. MARCH8 is the first example of an E3 ubiquitin ligase that is capable of inhibiting MHC II expression. Recent findings suggest that MARCH8 may regulate immune responses by promoting ubiquitination of MHC-II and CD86, leading to their subsequent endocytosis and lysosomal degradation.
• Synonyms: c-MIR; MARCH-VIII; MIR; RNF178

Reference Data

• Protein Families: Druggable Genome, Transmembrane