EBI3 Rabbit Polyclonal Antibody

CAT#: TA306701

Rabbit Polyclonal EBI3 Antibody

Product Images

Western blot analysis of EBI3 in K562 lysate with EBI3 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.

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  Immunohistochemistry of EBI3 in human spleen tissue with EBI3 antibody at 2.5 ug/ml.

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  Immunofluorescence of EBI3 in Human Spleen cells with EBI3 antibody at 20 ug/mL.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1 ug/mL, ICC: 2.5 ug/mL, IF: 20 ug/mL
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: EBI3 antibody was raised against a 16 amino acid peptide from near the carboxy terminus of human EBI3.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1ug/ul
• Purification: Affinity chromatography purified via peptide column
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Epstein-Barr virus induced 3
• Database Link:
  NP_005746
  Entrez Gene 50498 MouseEntrez Gene 10148 Human
  UniProt ID Q14213
• Background: EBI3 is a subunit in two distinct heterodimeric cytokines: interleukin-27 (IL-27) and IL-35 (1-3). Like interleukin-23 (IL-23), IL-27 is a recently discovered member of the IL-6/IL-12 family of proinflammatory and immunoregulatory cytokines. It exists as a heterodimer composed of the p40-related protein EBI3 and an IL-12 p35-related protein termed p28. IL-27 is produced after activation by antigen-presenting cells and induces proliferation of naïve but not memory CD4+ T-cells. It acts by binding to its receptor WSX-1 (also known as TCCR) and gp130 which results in the activation of a Jak/STAT signaling cascade, suggesting the IL-27 is involved in the regulation of immune processes. It has been suggested that IL-27 can also be used as a therapeutic agent against cancer as it can also induce tumor-specific anti-tumor activity mediated through CD8+ T-cells, IFN-g, and T-bet. IL-35 is composed of EBI3 and the p35 subunit of IL-12 and has been reported to have therapeutic effects against collagen-induced arthritis by expanding the population of regulatory T cells and suppressing Th17 cells. At least two isoform of EBI3 are known to exist.
• Synonyms: IL-27B; IL27B

Reference Data

• Protein Families: Druggable Genome, Secreted Protein