BORA Rabbit Polyclonal Antibody

CAT#: TA306752

Rabbit Polyclonal Bora Antibody

Product Images

Western blot analysis of Bora in mouse brain tissue lysate with Bora antibody at (A) 1 and (B) 2 ug/ml.

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  Immunohistochemistry of Bora in mouse brain tissue with Bora antibody at 2.5 ug/ml.

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  Immunofluorescence of Bora in Mouse Brain cells with Bora antibody at 20 ug/mL.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1 - 2 ug/mL, ICC: 2.5 ug/mL, IF: 20 ug/mL
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Bora antibody was raised against a 15 amino acid peptide from near the carboxy terminus of human Bora.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1ug/ul
• Purification: Affinity chromatography purified via peptide column
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: bora, aurora kinase A activator
• Database Link:
  NP_079084
  Entrez Gene 77744 MouseEntrez Gene 306102 RatEntrez Gene 79866 Human
  UniProt ID Q6PGQ7
• Background: Bora (Protein aurora borealis) is a key activator of Aurora Related Protein Kinase A (ARK-1), which is a centrosome-associated serine/threonine kinase that regulates centrosome maturation, bipolar spindle assembly and chromosome segregation during mitosis. Bora is localized to the nucleus until mitosis is initiated, then translocates to the cytoplasm in a Cdc2 dependent manner. Activation of Cdc2 initiates the release of Bora into the cytoplasm where it can bind and activate ARK-1. PLK1 (polo-like kinase-1) interacts with Bora to control the accessibility of its activation loop for phosphorylation and activation by ARK-1. Bora and ARK-1 cooperatively activate PLK1 and control mitotic entry. Bora mutants result in multipolar spindles in mitosis identical to those observed when ARK-1 function is blocked. Thus, the ARK1-Bora-PLK1 regulatory circuit in mammalian cells elucidates a key mechanism in cell cycle regulation. At least three isoforms of Bora are known to exist.
• Synonyms: C13orf34

Reference Data

• Protein Families: Druggable Genome