NOTUM Rabbit Polyclonal Antibody

CAT#: TA306819

Rabbit Polyclonal NOTUM Antibody

Product Images

Western blot analysis of NOTUM in human kidney tissue lysate with NOTUM antibody at (A) 0.5 and (B) 1 ug/ml.

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  Immunohistochemistry of NOTUM in human brain tissue with NOTUM antibody at 2.5 ug/ml.

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  Immunofluorescence of NOTUM in Mouse Kidney cells with NOTUM antibody at 20 ug/mL.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 0.5 - 1 ug/mL, ICC: 2.5 ug/mL, IF: 20 ug/mL
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: NOTUM antibody was raised against a 19 amino acid peptide near the amino terminus of human NOTUM.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Affinity chromatography purified via peptide column
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: NOTUM, palmitoleoyl-protein carboxylesterase
• Database Link:
  NP_848588
  Entrez Gene 77583 MouseEntrez Gene 303743 RatEntrez Gene 147111 Human
  UniProt ID Q6P988
• Background: In Drosophila, the notum gene is regulated by the Wingless pathway and encodes a secreted hydrolase that modifies heparan sulfate proteoglycans. The mammalian homolog has been shown to be able to cleave glypicans and can release GPI-anchored proteins from the mammalian cell surface. Like the Drosophilia NOTUM, the mammalian protein can act as a negative regulator of the Wnt signaling pathway. NOTUM is expressed at a low level in most mammalian tissues, although it is overexpressed in a subset of human hepatocellular carcinomas. Its transcription is regulated by b-catenin/TCF and is a target of the Wnt signaling pathway, forming a negative feedback loop that regulates the expression and activity of the Wnt pathway.
• Synonyms: hNOTUM