SQSTM1 Rabbit Polyclonal Antibody

CAT#: TA306861

Rabbit Polyclonal SQSTM1 Antibody

Product Images

Western blot analysis of SQSTM1 in Human spleen tissue lysate with SQSTM1 antibody at (A) 1 and (B) 2ug/ml.

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  Western blot analysis of SQSTM1 expression in K562 cell lysate with AP3B2 antibody at 1ug/ml in (A) the absence and (B) the presence of blocking peptide.

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  Immunohistochemistry of SQSTM1 in human spleen tissue with SQSTM1 antibody at 5ug/ml.

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  Immunohistochemistry of SQSTM1 in mouse spleen tissue with SQSTM1 antibody at 2ug/ml.

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  Immunohistochemistry of SQSTM1 in rat spleen tissue with SQSTM1 antibody at 5ug/ml.

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  Immunofluorescence of SQSTM1 in Rat Spleen cells with SQSTM1 antibody at 20ug/ml.

• Immunofluorescence of SQSTM1 in A431 cells with SQSTM1 antibody at 20ug/ml.

• Immunofluorescence of SQSTM1 in mouse spleen tissue with SQSTM1 antibody at 20ug/ml.

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Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1 - 2 ug/mL, ICC: 5 ug/mL, IF: 20 ug/mL
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: SQSTM1 antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human SQSTM1. The immunogen is located within the last 50 amino acids of SQSTM1.
• Formulation: PBS containing 0.02% sodium azide.
• Purification: Affinity chromatography purified via peptide column
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: sequestosome 1
• Database Link:
  NP_001135770
  Entrez Gene 18412 MouseEntrez Gene 113894 RatEntrez Gene 8878 Human
  UniProt ID Q13501
• Background: SQSTM1/p62 is an adapter protein which binds ubiquitin and regulates signaling cascades through ubiquitination. It may regulate the activation of NF-kappaB by TNF-alpha, nerve growth factor (NGF) and interleukin-1. SQSTM1/p62, a co-interacting protein of the atypical PKC isoforms, has a UBA domain at its C-terminal end, which binds non-covalently to polyubiquitin chain. SQSTM1’s UBA domain is necessary for recruitment of polyubiquitin and aggresome formation. SQSTM1 may play a role in titin/TTN downstream signaling in muscle cells and may be involved in cell differentiation, apoptosis, immune response and regulation of K+ channels. Mutations in the ubiquitin-associated (UBA) domain of SQSTM1 commonly cause Paget’s disease of bone since the UBA is necessary for aggregate sequestration and cell survival.
• Synonyms: A170; OSIL; p60; p62; p62B; PDB3; ZIP3

Reference Data

• Protein Families: Druggable Genome, Transcription Factors