Cytokeratin 4 (KRT4) Rabbit Monoclonal Antibody [Clone ID: EPR1598]

CAT#: TA307460

Rabbit monoclonal antibody against Cytokeratin 4(clone EPR1598)

Product Images

Western blot - Cytokeratin 4 antibody [EPR1598]; All lanes : Anti-Cytokeratin 4 antibody [EPR1598] at 1/1000 dilution.Lane 1 : A431 cell lysate.Lane 2 : Human uterus cell lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit IgG at 1/2000 dilution.Predicted band size : 57 kDa.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cytokeratin 4 antibody [EPR1598]; TA307460, at a 1/100 dilution, staining Cytokeratin 4 in paraffin embedded Human tonsil tissue by Immunohistochemistry. Note positive staining of squamous cells.

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  Immunocytochemistry/ Immunofluorescence - Cytokeratin 4 antibody [EPR1598]; Immunofluorescent staining of A431 cells, using TA307460 at a 1/100 dilution.

Specifications

Product Data

• Clone Name: EPR1598
• Applications: IF, IHC, WB
• Recommended Dilution: ICC: 1: 100 - 250, IHC: 1: 100 - 250, WB: 1: 1,000 - 10,000
• Reactivities: Human, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide corresponding to residues in human Cytokeratin 4 was used as an immunogen.
• Formulation: PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• Purification: Tissue culture supernatant
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 57 kDa
• Gene Name: keratin 4
• Database Link:
  NP_002263
  Entrez Gene 16682 MouseEntrez Gene 315323 RatEntrez Gene 3851 Human
  UniProt ID P19013
• Background: Keratins are a family of structurally related proteins that form the intermediate filament cytoskeleton in epithelial cells (1). Keratins are expressed in pairs by epithelial cells in a tissue and cell specific manner (2). The major differentiation specific keratins of the buccal mucosa, nasal, esophageal and anogenital epithelia are cytokeratin 4 and cytokeratn 13 (2). Cytokeratin 4 and cytokeratin 13 form heterodimers in normal epithelial cells (3). In humans, cytokeratin 4 is present in all layers of the epidermis at 10 weeks and gradually disappears (3). Genetic mutations in cytokeratin 4 cause white sponge nevus (WSN), an autosomal dominant disorder that affects noncornified stratified squamous epithelia (4).
• Synonyms: CK-4; CK4; CYK4; K4; WSN1
• Note: Does not react with Mouse