DNMT1 Rabbit Monoclonal Antibody [Clone ID: EPR3521(2)]

CAT#: TA307586

Rabbit monoclonal antibody against Dnmt1(clone EPR3521(2))

Product Images

Western blot - Anti-Dnmt1 antibody [EPR3521 (2)]; All lanes : Anti-Dnmt1 antibody [EPR3521 (2)] at 1/1000 dilution.Lane 1 : Jurkat cell lysates.Lane 2 : 293T cell lysates.Lane 3 : HeLa cell lysates.Lane 4 : HuT-78 cell lysates.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 183 kDa.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt1 antibody [EPR3521 (2)]; Immunohistochemical analysis of paraffin embedded Human stomach tissue labelled with TA307586 at 1/250 dilution.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt1 antibody [EPR3521 (2)]; Immunohistochemical analysis of paraffin embedded Human tonsil tissue labelled with TA307586 at 1/250 dilution.

Specifications

Product Data

• Clone Name: EPR3521(2)
• Applications: IHC, WB
• Recommended Dilution: WB: 1:1000 - 1:10000; IHC-P: 1:250 - 1:500; FC: 1:10 - 1:100
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide corresponding to residues in human Dnmt1 was used as an immunogen.
• Formulation: pH: 7.40Preservative: 0.01% Sodium azide; Constituents: 50% Glycerol, 0.05% BSA
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: DNA (cytosine-5-)-methyltransferase 1
• Database Link:
  NP_001124295
  Entrez Gene 13433 MouseEntrez Gene 84350 RatEntrez Gene 1786 Human
  UniProt ID P26358
• Background: Dnmt1 is one of three families of enzymes (Dnmt1, Dnmt2, Dnmt3 (a and b)) that have a role in the establishment and regulation of tissue-specific patterns of methylated cytosine residues. Abnormal methylation patterns are associated with certain human tumors and developmental abnormalities (1). Dnmt1 is responsible for maintenance of the DNA methylation pattern after DNA replication. Dnmt1 is a large protein and can be subdivided into an N- and a C-terminal part. The C-terminal domain harbors the catalytic center. The N-terminal part of the enzyme comprises several individual domains, and plays a role in intracellular targeting and regulation of the enzyme's activity. Dnmt1 shows a preference for hemimethylated CG sites and the specificity of Dnmt1 for hemimethylated target sites is a key property for that function (2).
• Synonyms: ADCADN; AIM; CXXC9; DNMT; HSN1E; m.HsaI; MCMT
• Note: Is unsuitable for ICC/IF or IP.

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Cysteine and methionine metabolism, Metabolic pathways