Epac1 (RAPGEF3) Rabbit Monoclonal Antibody [Clone ID: EPR1672]

CAT#: TA307796

Rabbit monoclonal antibody against Epac1(clone EPR1672)

Product Images

Western blot - Epac1 antibody [EPR1672]; All lanes : Anti-Epac1 antibody [EPR1672] at 1/1000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : HepG2 cell lysate.Lane 3 : Human heart lysate.Lane 4 : HUVEC cell lysate.Lysates/proteins at 10 ug per lane.Predicted band size : 104 kDa.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Epac1 antibody [EPR1672]; Immunohistochemical analysis of Epac1 in paraffin-embedded Human kidney tissue using TA307796 at 1/50 dilution.

Specifications

Product Data

• Clone Name: EPR1672
• Applications: IHC, WB
• Recommended Dilution: WB: 1:1000 - 1:10000; IP: 1:10 - 1:100; IHC-P: 1:50 - 1:100; ICC: 1:50 - 1:100
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide corresponding to residues in C-terminus of human Epac1 was used as an immunogen.
• Formulation: PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• Purification: Tissue culture supernatant
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 104 kDa
• Gene Name: Rap guanine nucleotide exchange factor 3
• Database Link:
  NP_006096
  Entrez Gene 59326 RatEntrez Gene 223864 MouseEntrez Gene 10411 Human
  UniProt ID O95398
• Background: Epac1 is a guanine nucleotide exchange factor (GEF) for the small G protein Rap and is directly activated by cyclic AMP (cAMP). Upon cAMP binding, Epac1 undergoes a conformational change that allows the interaction of its GEF domain with Rap, resulting in Rap activation and subsequent downstream effects, including integrin-mediated cell adhesion and cell-cell junction formation (1). In addition, activation of Epac1 has been shown to promote cell adhesion and polarization and enhance chemokine-induced migration (2).
• Synonyms: bcm910; CAMP-GEFI; EPAC; EPAC1; HSU79275
• Note: Is unsuitable for Flow Cyt.

Reference Data

• Protein Pathways: Leukocyte transendothelial migration, Long-term potentiation