SPR Rabbit Polyclonal Antibody

CAT#: TA307911

Rabbit Polyclonal antibody to SPR (sepiapterin reductase (7,8-dihydrobiopterin:NADP+ oxidoreductase))

Product Images

Sample (30 ug of whole cell lysate). A: A431. 12% SDS PAGE. TA307911 diluted at 1:1000.

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  SPR antibody [N2C3] detects SPR protein at cytosol on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. SPR antibody [N2C3] (TA307911) dilution: 1:500.

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  Immunofluorescence analysis of methanol-fixed HeLa, using SPR (TA307911) antibody at 1:200 dilution.

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  SPR antibody [N2C3] immunoprecipitates SPR protein in IP experiments. IP samples: A431 whole cell extract. A. Control with 4 ug of preimmune Rabbit IgG. B. Immunoprecipitation of SPR protein by 4 ug SPR antibody [N2C3] (TA307911). 5 % SDS-PAGE. The immuno

Specifications

Product Data

• Applications: IF, IHC, IP, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:3000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment corresponding to a region within amino acids 65 and 261 of SPR (Uniprot ID#P35270)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 28 kDa
• Gene Name: sepiapterin reductase (7,8-dihydrobiopterin:NADP+ oxidoreductase)
• Database Link:
  NP_003115
  Entrez Gene 6697 Human
  UniProt ID P35270
• Background: This gene encodes an aldo-keto reductase that catalyzes the NADPH-dependent reduction of pteridine derivatives and is important in the biosynthesis of tetrahydrobiopterin (BH4). Mutations in this gene result in DOPA-responsive dystonia due to sepiaterin reductase deficiency. A pseudogene has been identified on chromosome 1. [provided by RefSeq]
• Synonyms: SDR38C1
• Note: Seq homology of immunogen across species: Bovine (82%)

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Folate biosynthesis, Metabolic pathways