NANS Rabbit Polyclonal Antibody

CAT#: TA307925

Rabbit polyclonal antibody to NANS (N-acetylneuraminic acid synthase)

Product Images

Sample (30 ug of whole cell lysate). A: Hep G2. 10% SDS PAGE. NANS antibody. TA307925 diluted at 1:1000.

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  Immunohistochemical analysis of paraffin-embedded normal gastric epithelium (gland), using NANS (TA307925) antibody at 1:100 dilution.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: IHC:1:100-1:1000; WB:1:500-1:3000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment corresponding to a region within amino acids 54 and 359 of NANS (Uniprot ID#Q9NR45)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 40 kDa
• Gene Name: N-acetylneuraminate synthase
• Database Link:
  NP_061819
  Entrez Gene 94181 MouseEntrez Gene 298071 RatEntrez Gene 54187 Human
  UniProt ID Q9NR45
• Background: This gene encodes an enzyme that functions in the biosynthetic pathways of sialic acids. In vitro, the encoded protein uses N-acetylmannosamine 6-phosphate and mannose 6-phosphate as substrates to generate phosphorylated forms of N-acetylneuraminic acid (Neu5Ac) and 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN), respectively; however, it exhibits much higher activity toward the Neu5Ac phosphate product. In insect cells, expression of this gene results in Neu5Ac and KDN production. This gene is related to the E. coli sialic acid synthase gene neuB, and it can partially restore sialic acid synthase activity in an E. coli neuB-negative mutant. [provided by RefSeq]
• Synonyms: HEL-S-100; SAS
• Note: Seq homology of immunogen across species: Mouse (97%), Chicken (88%), Pig (97%), Rat (97%), Bovine (97%)

Reference Data

• Protein Pathways: Amino sugar and nucleotide sugar metabolism, Metabolic pathways