DCTN1 Rabbit Polyclonal Antibody

CAT#: TA307941

Rabbit polyclonal antibody to dynactin 1 (dynactin 1 (p150, glued homolog, Drosophila))

Product Images

Sample (30 ug of whole cell lysate). A: JurKat. 5% SDS PAGE. TA307941 diluted at 1:3000.

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  Immunohistochemical analysis of paraffin-embedded OVCA, using dynactin 1 (TA307941) antibody at 1:100 dilution.

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  Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using DCTN1 (TA307941) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with anti-alpha tubulin antibody (Red) at 1:2000.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:3000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to a region within amino acids 1216 and 1278 of DCTN1 (Uniprot ID#Q14203)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 142 kDa
• Gene Name: dynactin subunit 1
• Database Link:
  NP_001128513
  Entrez Gene 13191 MouseEntrez Gene 29167 RatEntrez Gene 1639 Human
  UniProt ID Q14203
• Background: This gene encodes the largest subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. Dynactin is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit interacts with dynein intermediate chain by its domains directly binding to dynein and binds to microtubules via a highly conserved glycine-rich cytoskeleton-associated protein (CAP-Gly) domain in its N-terminus. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. Mutations in this gene cause distal hereditary motor neuronopathy type VIIB (HMN7B) which is also known as distal spinal and bulbar muscular atrophy (dSBMA). [provided by RefSeq]
• Synonyms: DAP-150; DP-150; P135
• Note: Seq homology of immunogen across species: Mouse (100%), Rat (92%), Bovine (92%)

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Huntington's disease