FH Rabbit Polyclonal Antibody

CAT#: TA307996

Rabbit polyclonal antibody to fumarate hydratase (fumarate hydratase)

Product Images

Fumarate hydratase antibody detects FH protein by Western blot analysis. A. 50 ug mouse liver lysate/extract. 7.5% SDS-PAGE. Fumarate hydratase antibody (TA307996) dilution: 1:1000.

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  Fumarate hydratase antibody detects Fumarate hydratase protein by Western blot analysis. A.50 ug rat liver lysate/extract. 10 % SDS-PAGE. Fumarate hydratase antibody (TA307996) dilution: 1:5000

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  Sample (30 ug of whole cell lysate). A: HeLa. B: Hep G2. 10% SDS PAGE. TA307996 diluted at 1:1000

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  Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using fumarate hydratase (TA307996) antibody at 1:500 dilution.

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  Fumarate hydratase antibody detects Fumarate hydratase protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37°C for 30min. Green: Fumarate hydratase protein stained by Fumarate hyd

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Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:1000-1:10000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment corresponding to a region within amino acids 195 and 473 of fumarate hydratase (Uniprot ID#P07954)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 55 kDa
• Gene Name: fumarate hydratase
• Database Link:
  NP_000134
  Entrez Gene 2271 Human
  UniProt ID P07954
• Background: The protein encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy. [provided by RefSeq]
• Synonyms: FMRD; HLRCC; LRCC; MCL; MCUL1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Citrate cycle (TCA cycle), Metabolic pathways, Pathways in cancer, Renal cell carcinoma