Argininosuccinate Lyase (ASL) Rabbit Polyclonal Antibody

CAT#: TA308132

Rabbit Polyclonal antibody to ASL (argininosuccinate lyase)

Product Images

Sample (30 ug of whole cell lysate). A: MOLT4. 10% SDS PAGE. TA308132 diluted at 1:1000

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  Immunohistochemical analysis of paraffin-embedded DLD1 xenograft, using ASL (TA308132) antibody at 1:500 dilution.

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  ASL antibody detects ASL protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ASL protein stained by ASL antibody (TA308132) diluted at 1:500. Blue: Hoechst 33342 sta

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:3000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein fragment contain a sequence corresponding to a region within amino acids 86 and 325 of ASL
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 49 kDa
• Gene Name: argininosuccinate lyase
• Database Link:
  NP_000039
  Entrez Gene 435 Human
  UniProt ID P04424
• Background: This gene encodes a member of the lyase 1 family. The encoded protein forms a cytosolic homotetramer and primarily catalyzes the reversible hydrolytic cleavage of argininosuccinate into arginine and fumarate, an essential step in the liver in detoxifying ammonia via the urea cycle. Mutations in this gene result in the autosomal recessive disorder argininosuccinic aciduria, or argininosuccinic acid lyase deficiency. A nontranscribed pseudogene is also located on the long arm of chromosome 22. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq]
• Synonyms: ASAL

Reference Data

• Protein Pathways: Alanine, aspartate and glutamate metabolism, Arginine and proline metabolism, Metabolic pathways