MRPS5 Rabbit Polyclonal Antibody

CAT#: TA308181

Rabbit Polyclonal antibody to MRPS5 (mitochondrial ribosomal protein S5)

Product Images

Sample (30 ug whole cell lysate). A:A431. B:H1299. C:HeLa S3. D:Hep G2. 10% SDS PAGE. TA308181 diluted at 1:1000

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  MRPS5 antibody detects MRPS5 protein at cytosol on AGS xenograft by immunohistochemical analysis. Sample: Paraffin-embedded AGS xenograft. MRPS5 antibody (TA308181) dilution: 1:500.

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  Immunofluorescence analysis of methanol-fixed HeLa, using MRPS5 (TA308181) antibody at 1:200 dilution.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:3000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 430 of MRPS5 (Uniprot ID#P82675)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 48 kDa
• Gene Name: mitochondrial ribosomal protein S5
• Database Link:
  NP_114108
  Entrez Gene 64969 Human
  UniProt ID P82675
• Background: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S5P family. Pseudogenes corresponding to this gene are found on chromosomes 4q, 5q, and 18q. [provided by RefSeq]
• Synonyms: MRP-S5; S5mt