TPP1 Rabbit Polyclonal Antibody

CAT#: TA308203

Rabbit Polyclonal antibody to TPP1 (tripeptidyl peptidase I)

Product Images

Sample (30 ug of whole cell lysate). A: A431. B: H1299. C: Hela. 7.5% SDS PAGE. TA308203 diluted at 1:1000.

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  Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using TPP1 (TA308203) antibody at 1:100 dilution.

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  Immunofluorescence analysis of methanol-fixed A431, using TPP1 (TA308203) antibody at 1:200 dilution.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:3000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment corresponding to a region within amino acids 66 and 324 of TPP1 (Uniprot ID#O14773)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 61 kDa
• Gene Name: tripeptidyl peptidase 1
• Database Link:
  NP_000382
  Entrez Gene 1200 Human
  UniProt ID O14773
• Background: This gene encodes a member of the sedolisin family of serine proteases. The protease functions in the lysosome to cleave N-terminal tripeptides from substrates, and has weaker endopeptidase activity. It is synthesized as a catalytically-inactive enzyme which is activated and auto-proteolyzed upon acidification. Mutations in this gene result in late-infantile neuronal ceroid lipofuscinosis, which is associated with the failure to degrade specific neuropeptides and a subunit of ATP synthase in the lysosome. [provided by RefSeq]
• Synonyms: CLN2; GIG1; LPIC; SCAR7; TPP-1
• Note: Seq homology of immunogen across species: Chimpanzee (100%)

Reference Data

• Protein Families: Protease
• Protein Pathways: Lysosome