OGT Rabbit Polyclonal Antibody

CAT#: TA308359

Rabbit Polyclonal antibody to O-GlcNAc transferase (O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase))

Product Images

O-GlcNAc transferase antibody detects O-GlcNAc transferase protein by western blot analysis. A. 30 ug 293T whole cell extract. B. 30 ug A431 whole cell extract. C. 30 ug HeLa whole cell extract. D. 30 ug HepG2 whole cell extract. 7.5 % SDS-PAGE. O-GlcNAc

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  O-GlcNAc transferase antibody detects O-GlcNAc transferase protein by western blot analysis. A. 50 ug mouse brain extract. 5 % SDS-PAGE. O-GlcNAc transferase antibody (TA308359) dilution: 1:1000

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  O-GlcNAc transferase antibody detects O-GlcNAc transferase protein by western blot analysis. A. 30 ug PC-12 whole cell extract. 5 % SDS-PAGE. O-GlcNAc transferase antibody (TA308359) dilution: 1:1000

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  Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft, using O-GlcNAc transferase (TA308359) antibody at 1:100 dilution.

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  Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using O-GlcNAc transferase (TA308359) antibody at 1:200 dilution.

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Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:3000
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment corresponding to a region within amino acids 223 and 502 of O-GlcNAc transferase (Uniprot ID#O15294)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 116 kDa
• Gene Name: O-linked N-acetylglucosamine (GlcNAc) transferase
• Database Link:
  NP_858059
  Entrez Gene 108155 MouseEntrez Gene 8473 Human
  UniProt ID O15294
• Background: O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT) catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The protein contains nine tetratricopeptide repeats and a putative bipartite nuclear localization signal. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq]
• Synonyms: HRNT1; O-GLCNAC
• Note: Seq homology of immunogen across species: Pig (100%), Bovine (100%)

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, O-Glycan biosynthesis