NSL1 Rabbit Polyclonal Antibody

CAT#: TA308390

Rabbit polyclonal antibody to DC8 (DC8, MIND kinetochore complex component, homolog (S. cerevisiae))

Product Images

DC8 antibody detects NSL1 protein by Western blot analysis. A. 30 ug C8D30 whole cell lysate/extract. 12 % SDS-PAGE. DC8 antibody (TA308390) dilution: 1:1000

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  Sample (30 ug of whole cell lysate). A:Hep G2. 12% SDS PAGE. TA308390 diluted at 1:1000

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  DC8 antibody detects NSL1 protein by Western blot analysis. A. 30 ug Rat2 whole cell lysate/extract. 12 % SDS-PAGE. DC8 antibody (TA308390) dilution: 1:1000

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  Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using DC8 (TA308390) antibody at 1:200 dilution.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; WB:1:500-1:3000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 275 of DC8 (Uniprot ID#Q96IY1)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 32 kDa
• Gene Name: NSL1, MIS12 kinetochore complex component
• Database Link:
  NP_001036014
  Entrez Gene 25936 Human
  UniProt ID Q96IY1
• Background: This gene encodes a protein with two coiled-coil domains that localizes to kinetochores, which are chromosome-associated structures that attach to microtubules and mediate chromosome movements during cell division. The encoded protein is part of a conserved protein complex that includes two chromodomain-containing proteins and a component of the outer plate of the kinetochore. This protein complex is proposed to bridge centromeric heterochromatin with the outer kinetochore structure. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
• Synonyms: C1orf48; DC8; MIS14