P4HB Rabbit Polyclonal Antibody

CAT#: TA308403

Rabbit Polyclonal antibody to PDI (prolyl 4-hydroxylase, beta polypeptide)

Product Images

Sample (20 ug of whole cell lysate). A: mouse liver. 7.5% SDS PAGE. TA308403 diluted at 1:10000.

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  Sample (30 ug of whole cell lysate). A:293T. B:A431. C:H1299. 7.5% SDS PAGE. TA308403 diluted at 1:500

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  Immunohistochemical analysis of paraffin-embedded H520 xenograft, using PDI (TA308403) antibody at 1:100 dilution.

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  PDI antibody [N1N3] detects PDI protein at cytosol and membrane on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. PDI antibody [N1N3] (TA308403) dilution: 1:500.

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  Immunofluorescence analysis of methanol-fixed A431, using PDI (TA308403) antibody at 1:200 dilution.

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Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:10000
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment corresponding to a region within amino acids 23 and 227 of PDI (Uniprot ID#P07237)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 57 kDa
• Gene Name: prolyl 4-hydroxylase subunit beta
• Database Link:
  NP_000909
  Entrez Gene 18453 MouseEntrez Gene 5034 Human
  UniProt ID P07237
• Background: This gene encodes the beta subunit of prolyl 4-hydroxylase, a highly abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen. This enzyme is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex. [provided by RefSeq]
• Synonyms: DSI; ERBA2L; GIT; P4Hbeta; PDI; PDIA1; PHDB; PO4DB; PO4HB; PROHB
• Note: Seq homology of immunogen across species: Chimpanzee (100%)

Reference Data

• Protein Families: Druggable Genome