TRIM32 Rabbit Polyclonal Antibody

CAT#: TA308451

Rabbit polyclonal antibody to TRIM32 (tripartite motif-containing 32)

Product Images

TRIM32 antibody detects TRIM32 protein by western blot analysis. A. 30 ug 293T whole cell lysate/extract. 7.5 % SDS-PAGE. TRIM32 antibody (TA308451) dilution: 1:1000

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  TRIM32 antibody detects TRIM32 protein by western blot analysis. A. 5 ug 293T whole cell lysate/extract. B. 5 ug whole cell lysate/extract of DDDDK-human TRIM32-transfected 293T cells. 7.5 % SDS-PAGE. TRIM32 antibody (TA308451) dilution: 1:20000

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  TRIM32 antibody detects TRIM32 protein at cytoplasm by immunofluorescent analysis. Sample: A375 cells were fixed in ice-cold MeOH for 5 min. Green: TRIM32 protein stained by TRIM32 antibody (TA308451) diluted at 1:500. Blue: Hoechst 33342 staining. Scale

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; WB:1:500-1:20000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 289 of TRIM32 (Uniprot ID#Q13049)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 72 kDa
• Gene Name: tripartite motif containing 32
• Database Link:
  NP_001093149
  Entrez Gene 22954 Human
  UniProt ID Q13049
• Background: The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic bodies. The protein has also been localized to the nucleus, where it interacts with the activation domain of the HIV-1 Tat protein. The Tat protein activates transcription of HIV-1 genes. [provided by RefSeq]
• Synonyms: BBS11; HT2A; LGMD2H; TATIP
• Note: Seq homology of immunogen across species: Rhesus Monkey (100%)

Reference Data

• Protein Families: Transcription Factors
• Protein Pathways: Ubiquitin mediated proteolysis