HHIP Rabbit Polyclonal Antibody

CAT#: TA308452

Rabbit Polyclonal antibody to HHIP (hedgehog interacting protein)

Product Images

Sample (50 ug of whole cell lysate). A: Mouse brain. 7.5% SDS PAGE. TA308452 diluted at 1:1000.

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  Sample (30 ug of whole cell lysate). A: A431. 7.5% SDS PAGE. TA308452 diluted at 1:1000.

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  HHIP antibody [N3C2], Internal detects HHIP protein at cytosol on hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. HHIP antibody [N3C2], Internal (TA308452) dilution: 1:500.

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  HHIP antibody [N3C2], Internal antibody detects HHIP protein by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde for 15 min. Green: HHIP protein stained by HHIP antibody [N3C2], Internal antibody (TA308452) diluted at 1:500

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: IHC:1:100-1:1000; WB:1:500-1:3000
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment corresponding to a region within amino acids 263 and 554 of HHIP (Uniprot ID#Q96QV1)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 79 kDa
• Gene Name: hedgehog interacting protein
• Database Link:
  NP_071920
  Entrez Gene 15245 MouseEntrez Gene 64399 Human
  UniProt ID Q96QV1
• Background: This gene encodes a protein similar to the mouse hedgehog-interacting protein, a regulatory component of the hedgehog signalling pathway. Members of the hedgehog family are evolutionarily conserved proteins which are involved in many fundamental processes in embryonic development, including anteroposterior patterns of limbs and regulation of left-right asymmetry. [provided by RefSeq]
• Synonyms: HIP
• Note: Seq homology of immunogen across species: Rhesus Monkey (100%)

Reference Data

• Protein Families: Secreted Protein
• Protein Pathways: Basal cell carcinoma, Hedgehog signaling pathway, Pathways in cancer