Pyruvate Kinase (PKLR) Rabbit Polyclonal Antibody

CAT#: TA308453

Rabbit Polyclonal antibody to Pyruvate Kinase (liver/RBC) (pyruvate kinase, liver and RBC)

Product Images

Sample (30 ug of whole cell lysate)Â. Â A: 293T. Â B: A431. Â C: HeLa. Â D: HepG2. Â E: A375. Â 7.5% SDS PAGEÂ. Â TA308453 diluted at 1:1000. Â Â

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  Pyruvate Kinase (liver/RBC) antibody detects Pyruvate Kinase (liver/RBC) protein by Western blot analysis. A. 20 ug Rat liver lysate/extract. 7.5% SDS-PAGE. Pyruvate Kinase (liver/RBC) antibody (TA308453) dilution: 1:10000.

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  Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using Pyruvate Kinase (liver/RBC) (TA308453) antibody at 1:500 dilution.

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  Pyruvate Kinase (liver/RBC) antibody detects PKLR protein at Cytoplasm by immunofluorescent analysis. Sample: HepG2 cells were fixed in -20â 100% MeOH for 5 min. Green: PKLR protein stained by Pyruvate Kinase (liver/RBC) antibody (TA308453) diluted at 1:

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:1000-1:10000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 260 of Pyruvate Kinase (liver/RBC) (Uniprot ID#P30613)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 62 kDa
• Gene Name: pyruvate kinase, liver and RBC
• Database Link:
  NP_870986
  Entrez Gene 5313 Human
  UniProt ID P30613
• Background: The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
• Synonyms: PK1; PKL; PKR; PKRL; RPK

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Glycolysis / Gluconeogenesis, Insulin signaling pathway, Maturity onset diabetes of the young, Metabolic pathways, Purine metabolism, Pyruvate metabolism, Type II diabetes mellitus