TJP1 Rabbit Polyclonal Antibody

CAT#: TA308455

Rabbit polyclonal antibody to ZO-1 (tight junction protein 1 (zona occludens 1))

Product Images

Sample (30 ug whole cell lysate). A:H1299. 5% SDS PAGE. TA308455 diluted at 1:3000

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  Immunohistochemical analysis of paraffin-embedded H661 xenograft, using ZO-1 (TA308455) antibody at 1:250 dilution.

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  ZO-1 antibody [N1N2], N-term detects TJP1 protein at junction by confocal immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TJP1 protein stained by ZO-1 antibody [N1N2], N-term (TA308455) diluted at

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:3000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 296 of ZO-1 (Uniprot ID#Q07157)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 187 kDa
• Gene Name: tight junction protein 1
• Database Link:
  NP_003248
  Entrez Gene 7082 Human
  UniProt ID Q07157
• Background: This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]
• Synonyms: ZO-1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Adherens junction, Epithelial cell signaling in Helicobacter pylori infection, Gap junction, Tight junction, Vibrio cholerae infection