Triosephosphate isomerase (TPI1) Rabbit Polyclonal Antibody

CAT#: TA308459

Rabbit Polyclonal antibody to Triosephosphate isomerase (triosephosphate isomerase 1)

Product Images

Triosephosphate isomerase antibody [C2C3], C-term detects TPI1 protein by Western blot analysis. A. 30 ug Neuro2A whole cell lysate/extract. B. 30 ug GL261 whole cell lysate/extract. C. 30 ug C8D30 whole cell lysate/extract. D. 30 ug NIH-3T3 whole cell ly

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  Triosephosphate isomerase antibody [C2C3], C-term detects TPI1 protein by Western blot analysis. A. 30 ug PC-12 whole cell lysate/extract. B. 30 ug Rat2 whole cell lysate/extract. 12 % SDS-PAGE. Triosephosphate isomerase antibody [C2C3], C-term (TA308459)

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  Sample (30 ug of whole cell lysate). A:A431. B:H1299. 12% SDS PAGE. TA308459 diluted at 1:2000

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  Immunohistochemical analysis of paraffin-embedded SNU-16 xenograft, using TPI1 (TA308459) antibody at 1:100 dilution.

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  Triosephosphate isomerase antibody [C2C3], C-term detects Triosephosphate isomerase protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Triosephosphate isomerase protein stained

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Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: ELISA:; ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:3000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to a region within amino acids 187 and 249 of Triosephosphate isomerase (Uniprot ID#P60174)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 27 kDa
• Gene Name: triosephosphate isomerase 1
• Database Link:
  NP_000356
  Entrez Gene 477711 DogEntrez Gene 7167 Human
  UniProt ID P60174
• Background: This gene encodes an enzyme, consisting of two identical proteins, which catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis. Mutations in this gene are associated with triosephosphate isomerase deficiency. Pseudogenes have been identified on chromosomes 1, 4, 6 and 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq]
• Synonyms: HEL-S-49; TIM; TPI; TPID
• Note: Seq homology of immunogen across species: Yeast (83%), Xenopus Tropicalis (92%), Dog (100%), Chicken (92%), Chimpanzee (100%), Bovine (100%)

Reference Data

• Protein Pathways: Fructose and mannose metabolism, Glycolysis / Gluconeogenesis, Inositol phosphate metabolism, Metabolic pathways