EEF2K Rabbit Polyclonal Antibody

CAT#: TA308475

Rabbit Polyclonal antibody to EEF2K (eukaryotic elongation factor-2 kinase)

Product Images

Sample (50 ug of whole cell lysate). A: mouse liver. 7.5% SDS PAGE. TA308475 diluted at 1:1000.

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  Sample (30 ug of whole cell lysate). A: A431. B: H1299. C: Hela. D: Hep G2. 7.5% SDS PAGE. TA308475 diluted at 1:5000.

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  Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using eEF-2K (TA308475) antibody at 1:500 dilution.

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  Immunofluorescence analysis of paraformaldehyde-fixed A431, using eEF-2K (TA308475) antibody at 1:200 dilution.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:1000-1:10000
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 268 of EEF2K (Uniprot ID#O00418)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 82 kDa
• Gene Name: eukaryotic elongation factor 2 kinase
• Database Link:
  NP_037434
  Entrez Gene 13631 MouseEntrez Gene 29904 Human
  UniProt ID O00418
• Background: This gene encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment. [provided by RefSeq]
• Synonyms: eEF-2K; HSU93850
• Note: Seq homology of immunogen across species: Bovine (90%)

Reference Data

• Protein Families: Druggable Genome, Protein Kinase