alpha Actinin 4 (ACTN4) Rabbit Polyclonal Antibody

CAT#: TA308476

Rabbit Polyclonal antibody to alpha Actinin 4 (actinin, alpha 4)

Product Images

Sample (50 ug of whole cell lysate). A: Mouse brain. 7.5% SDS PAGE. TA308476 diluted at 1:1000.

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  alpha Actinin 4 antibody [N2C1], Internal detects alpha Actinin 4 protein by Western blot analysis. A. 30 ug PC-12 whole cell lysate/extract. 7.5% SDS-PAGE. alpha Actinin 4 antibody [N2C1], Internal (TA308476) dilution: 1:3000.

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  Sample (30 ug of whole cell lysate). A: A431. B: Hep G2. 7.5% SDS PAGE. TA308476 diluted at 1:1000.

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  Sample (30 ug of whole cell lysate). A: NIH-3T3. 7.5% SDS PAGE. TA308476 diluted at 1:1000.

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  Immunohistochemical analysis of paraffin-embedded A549 xenograft, using alpha Actinin 4 (TA308476) antibody at 1:500 dilution.

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  Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using alpha Actinin 4 (TA308476) antibody at 1:200 dilution.

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Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:3000
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment corresponding to a region within amino acids 206 and 542 of alpha Actinin 4 (Uniprot ID#O43707)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 105 kDa
• Gene Name: actinin alpha 4
• Database Link:
  NP_004915
  Entrez Gene 60595 MouseEntrez Gene 81 Human
  UniProt ID O43707
• Background: Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis. [provided by RefSeq]
• Synonyms: ACTININ-4; FSGS; FSGS1
• Note: Seq homology of immunogen across species: Rhesus Monkey (100%), Bovine (100%)

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton, Systemic lupus erythematosus, Tight junction