Cofilin 2 (CFL2) Rabbit Polyclonal Antibody

CAT#: TA308500

Rabbit Polyclonal antibody to Cofilin 2 (muscle) (cofilin 2 (muscle))

Product Images

Sample (50 ug of whole cell lysate). A: mouse brain. 12% SDS PAGE. TA308500 diluted at 1:1000.

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  Sample (30 ug of whole cell lysate). A: H1299. B: Molt-4. 12% SDS PAGE. TA308500 diluted at 1:1000.

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  Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Cofilin 2 (muscle) (TA308500) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with anti-alpha tubulin antibody (Red) at 1:2500.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; WB:1:500-1:3000
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 166 of Cofilin 2 (muscle) (Uniprot ID#Q9Y281)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 19 kDa
• Gene Name: cofilin 2
• Database Link:
  NP_068733
  Entrez Gene 12632 MouseEntrez Gene 1073 Human
  UniProt ID Q9Y281
• Background: Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. (Gillett et al., 1996 [PubMed 8800436]). Cofilin-2 is a member of the AC group of proteins that also includes cofilin-1 (CFL1) and destrin (DSTN; MIM 609114), all of which regulate actin-filament dynamics (Bamburg et al., 1999 [PubMed 10461190]; Maciver and Hussey, 2002). The CFL2 gene encodes a skeletal muscle-specific isoform (Vartiainen et al., 2002 [PubMed 11809832]) localized to the thin filaments, where it exerts its effect on actin, in part through interactions with tropomyosins (Ono and Ono, 2002 [PubMed 11901171]). [supplied by OMIM]
• Synonyms: NEM7
• Note: Seq homology of immunogen across species: Pig (100%), Rhesus Monkey (100%), Bovine (100%)

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Axon guidance, Fc gamma R-mediated phagocytosis, Regulation of actin cytoskeleton