BCCIP Rabbit Polyclonal Antibody

CAT#: TA308557

Rabbit polyclonal antibody to TOK-1 alpha (BRCA2 and CDKN1A interacting protein)

Product Images

Sample (30 ug of whole cell lysate). A: A431. B: Hela. 10% SDS PAGE. TA308557 diluted at 1:1000.

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  BCCIP antibody detects BCCIP protein at nucleus on BT483 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded BT483 xenograft. BCCIP antibody (TA308557) dilution: 1:500.

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  BCCIP antibody detects BCCIP protein at nucleus on U373 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U373 xenograft. BCCIP antibody (TA308557) dilution: 1:500.

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  Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using BCCIP (TA308557) antibody at 1:200 dilution.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:3000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to a region within amino acids 181 and 273 of BCCIP (Uniprot ID#Q9P287)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 36 kDa
• Gene Name: BRCA2 and CDKN1A interacting protein
• Database Link:
  NP_057651
  Entrez Gene 56647 Human
  UniProt ID Q9P287
• Background: This gene product was isolated on the basis of its interaction with BRCA2 and p21 proteins. It is an evolutionarily conserved nuclear protein with multiple interacting domains. The N-terminal half shares moderate homology with regions of calmodulin and M-calpain, suggesting that it may also bind calcium. Functional studies indicate that this protein may be an important cofactor for BRCA2 in tumor suppression, and a modulator of CDK2 kinase activity via p21. This protein has also been implicated in the regulation of BRCA2 and RAD51 nuclear focus formation, double-strand break-induced homologous recombination, and cell cycle progression. Multiple transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq]
• Synonyms: TOK-1; TOK1

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency