DIAPH1 Rabbit Polyclonal Antibody

CAT#: TA308571

Rabbit Polyclonal antibody to DIAPH1 (diaphanous homolog 1 (Drosophila))

Product Images

Sample (30 ug of whole cell lysate). A: H1299. B: Raji. 7.5% SDS PAGE. TA308571 diluted at 1:1000.

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  Immunohistochemical analysis of paraffin-embedded gastric tissue, using DIAPH1 (TA308571) antibody at 1:100 dilution.

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  Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using DIAPH1 (TA308571) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with anti-alpha tubulin antibody (Red) at 1:2000.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:3000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 237 of DIAPH1 (Uniprot ID#O60610)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 141 kDa
• Gene Name: diaphanous related formin 1
• Database Link:
  NP_005210
  Entrez Gene 1729 Human
  UniProt ID O60610
• Background: This gene is a homolog of the Drosophila diaphanous gene, and has been linked to autosomal dominant, fully penetrant, nonsyndromic sensorineural progressive low-frequency hearing loss. Actin polymerization involves proteins known to interact with diaphanous protein in Drosophila and mouse. It has therefore been speculated that this gene may have a role in the regulation of actin polymerization in hair cells of the inner ear. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq]
• Synonyms: DFNA1; DIA1; DRF1; hDIA1; LFHL1; SCBMS

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Focal adhesion, Regulation of actin cytoskeleton