ARF1 Rabbit Polyclonal Antibody

CAT#: TA308576

Rabbit Polyclonal antibody to ARF1 (ADP-ribosylation factor 1)

Product Images

Sample (50 ug of whole cell lysate). A: mouse brain. 12% SDS PAGE. TA308576 diluted at 1:10000.

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  Sample (30 ug of whole cell lysate). A: A431. B: H1299. 12% SDS PAGE. TA308576 diluted at 1:1000.

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  Immunohistochemical analysis of paraffin-embedded MDA-MB468 xenograft, using ARF1 (TA308576) antibody at 1:100 dilution.

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  Immunofluorescence analysis of methanol-fixed HeLa, using ARF1 (TA308576) antibody at 1:200 dilution.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:1000-1:10000
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment contain a sequence corresponding to a region within amino acids 1 and 162 of ARF1 (Uniprot ID#P84077)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 21 kDa
• Gene Name: ADP ribosylation factor 1
• Database Link:
  NP_001019399
  Entrez Gene 11840 MouseEntrez Gene 64310 RatEntrez Gene 106557448 DogEntrez Gene 100856517 DogEntrez Gene 375 Human
• Background: ADP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. The family members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D. The gene products, including 6 ARF proteins and 11 ARF-like proteins, constitute a family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6), and members of each class share a common gene organization. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]
• Synonyms: ADP-ribosylation factor 1; OTTHUMP00000035715
• Note: Seq homology of immunogen across species: Rat (100%), Dog (100%), Pig (100%), Sheep (100%), Rhesus Monkey (100%), Bovine (100%)

Reference Data

• Protein Pathways: Vibrio cholerae infection