PSMF1 Rabbit Polyclonal Antibody

CAT#: TA308605

Rabbit polyclonal antibody to Proteasome inhibitor PI31 subunit (proteasome (prosome, macropain) inhibitor subunit 1 (PI31))

Product Images

Sample (30 ug of whole cell lysate). A: Hep G2. 12% SDS PAGE. TA308605 diluted at 1:1000.

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  PSMF1 antibody [C2C3], C-term detects PSMF1 protein at Cytoplasm and Nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PSMF1 protein stained by PSMF1 antibody [C2C3], C-term (TA308605) dil

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; WB:1:500-1:3000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to a region within amino acids 209 and 271 of PSMF1 (Uniprot ID#Q92530)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 30 kDa
• Gene Name: proteasome inhibitor subunit 1
• Database Link:
  NP_848693
  Entrez Gene 228769 MouseEntrez Gene 689852 RatEntrez Gene 9491 Human
  UniProt ID Q92530
• Background: The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a protein that inhibits the activation of the proteasome by the 11S and 19S regulators. Alternative transcript variants have been identified for this gene. [provided by RefSeq]
• Synonyms: PI31
• Note: Seq homology of immunogen across species: Mouse (100%), Rat (100%), Pig (100%), Chicken (85%), Bovine (100%)

Reference Data

• Protein Pathways: Proteasome