PFKL Rabbit Polyclonal Antibody

CAT#: TA308631

Rabbit Polyclonal antibody to PFKL (phosphofructokinase, liver)

Product Images

Sample (30 ug of whole cell lysate). A: Hep G2. 7.5% SDS PAGE. TA308631 diluted at 1:1000

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  Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using PFKL (TA308631) antibody at 1:500 dilution.

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  Immunofluorescence analysis of paraformaldehyde-fixed A549, using PFKL (TA308631) antibody at 1:200 dilution.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:3000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment corresponding to a region within amino acids 428 and 674 of PFKL (Uniprot ID#P17858)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 90 kDa
• Gene Name: phosphofructokinase, liver type
• Database Link:
  NP_001002021
  Entrez Gene 18641 MouseEntrez Gene 25741 RatEntrez Gene 5211 Human
  UniProt ID P17858
• Background: Phosphofructokinase (PFK) is a tetrameric enzyme that catalyzes a key step in glycolysis, namely the conversion of D-fructose 6-phosphate to D-fructose 1,6-bisphosphate. Separate genes encode a muscle subunit (M) and a liver subunit (L). PFK from muscle is a homotetramer of M subunits, PFK from liver is a homotetramer of L-subunits, while PFK from platelets can be composed of any tetrameric combination of M and L subunits. The protein encoded by this gene represents the L subunit. Alternate splicing results in two transcript variants, one of which is a candidate for nonsense-mediated decay (NMD). [provided by RefSeq]
• Synonyms: ATP-PFK; PFK-B; PFK-L
• Note: Seq homology of immunogen across species: Mouse (95%), Rat (95%), Bovine (94%)

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Fructose and mannose metabolism, Galactose metabolism, Glycolysis / Gluconeogenesis, Metabolic pathways, Pentose phosphate pathway