PFKM Rabbit Polyclonal Antibody

CAT#: TA308655

Rabbit Polyclonal antibody to PFK (muscle) (phosphofructokinase, muscle)

Product Images

Sample (50 ug of whole cell lysate). A: Mouse brain. 7.5% SDS PAGE. TA308655 diluted at 1:2000.

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  Sample (30 ug of whole cell lysate). A: A431. 7.5% SDS PAGE. TA308655 diluted at 1:5000.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB:1:1000-1:10000
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 253 of PFK (muscle) (Uniprot ID#P08237)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 85 kDa
• Gene Name: phosphofructokinase, muscle
• Database Link:
  NP_000280
  Entrez Gene 18642 MouseEntrez Gene 65152 RatEntrez Gene 403849 DogEntrez Gene 5213 Human
  UniProt ID P08237
• Background: The PFKM gene encodes the muscle isoform of phosphofructokinase (PFK) (ATP:D-fructose-6-phosphate-1-phosphotransferase, EC 2.7.1.11). PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. Mammalian PFK is a tetramer made up of various combinations of 3 subunits: muscle (PFKM), liver (PFKL; MIM 171860), and platelet (PFKP; MIM 171840), the genes for which are located on chromosomes 12q13, 21q22, and 10p, respectively. The composition of the tetramers differs according to the tissue type. Muscle and liver PFK are a homotetramers of 4M and 4L subunits, respectively. Erythrocytes contain both L and M subunits, which randomly tetramerize to form M4, L4, and M3L, M2L2, and ML3 hybrid forms of the holoenzyme (Vora et al., 1980 [PubMed 6444721]; Raben and Sherman, 1995 [PubMed 7550225]). [supplied by OMIM]
• Synonyms: ATP-PFK; GSD7; PFK-1; PFK1; PFKA; PFKX; PPP1R122
• Note: Seq homology of immunogen across species: Chicken (88%), Dog (97%), Pig (95%), Rat (94%), Xenopus laevis (89%), Bovine (96%)

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Fructose and mannose metabolism, Galactose metabolism, Glycolysis / Gluconeogenesis, Metabolic pathways, Pentose phosphate pathway