PCMT1 Rabbit Polyclonal Antibody

CAT#: TA308667

Rabbit Polyclonal antibody to PCMT1 (protein-L-isoaspartate (D-aspartate) O-methyltransferase)

Product Images

Sample (30 ug of whole cell lysate). A: 293T. 12% SDS PAGE. TA308667 diluted at 1:1000.

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  Immunohistochemical analysis of paraffin-embedded U87 xenograft, using PCMT1 (TA308667) antibody at 1:500 dilution.

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  Immunofluorescence analysis of methanol-fixed A431, using PCMT1 (TA308667) antibody at 1:200 dilution.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:3000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein fragment contain a sequence corresponding to a region within amino acids 10 and 227 of PCMT1
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 25 kDa
• Gene Name: protein-L-isoaspartate (D-aspartate) O-methyltransferase
• Database Link:
  NP_005380
  Entrez Gene 5110 Human
  UniProt ID P22061
• Background: Three classes of protein carboxyl methyltransferases, distinguished by their methyl-acceptor substrate specificity, have been found in prokaryotic and eukaryotic cells. The type II enzyme catalyzes the transfer of a methyl group from S-adenosyl-L-methionine to the free carboxyl groups of D-aspartyl and L-isoaspartyl residues. These methyl-accepting residues result from the spontaneous deamidation, isomerization, and racemization of normal L-aspartyl and L-asparaginyl residues and represent sites of covalent damage to aging proteins PCMT1 (EC 2.1.1.77) is a protein repair enzyme that initiates the conversion of abnormal D-aspartyl and L-isoaspartyl residues to the normal L-aspartyl form. [supplied by OMIM]
• Synonyms: PIMT

Reference Data

• Protein Families: Druggable Genome