Alpha SNAP (NAPA) Rabbit Polyclonal Antibody

CAT#: TA308674

Rabbit Polyclonal antibody to alpha SNAP (N-ethylmaleimide-sensitive factor attachment protein, alpha)

Product Images

Sample (50 ug of whole cell lysate). A: mouse brain. 12% SDS PAGE. TA308674 diluted at 1:10000.

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  Sample (30 ug whole cell lysate). A:Hep G2. 10% SDS PAGE. TA308674 diluted at 1:1000

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  alpha SNAP antibody [N2C3] detects alpha SNAP protein at cytosol on mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. alpha SNAP antibody [N2C3] (TA308674) dilution: 1:500.

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  Immunofluorescence analysis of methanol-fixed A431, using alpha SNAP (TA308674) antibody at 1:200 dilution.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:1000-1:10000
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment corresponding to a region within amino acids 78 and 295 of alpha SNAP (Uniprot ID#P54920)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 33 kDa
• Gene Name: NSF attachment protein alpha
• Database Link:
  NP_003818
  Entrez Gene 108124 MouseEntrez Gene 8775 Human
  UniProt ID P54920
• Background: The 'SNARE hypothesis' is a model explaining the process of docking and fusion of vesicles to their target membranes. According to this model, membrane proteins from the vesicle (v-SNAREs) and proteins from the target membrane (t-SNAREs) govern the specificity of vesicle targeting and docking through mutual recognition. Once the 2 classes of SNAREs bind to each other, they form a complex that recruits the general elements of the fusion apparatus, namely NSF (N-ethylmaleimide-sensitive factor) and SNAPs (soluble NSF-attachment proteins), to the site of membrane fusion, thereby forming the 20S fusion complex. Alpha- and gamma-SNAP are found in a wide range of tissues and act synergistically in intra-Golgi transport. The sequence of the predicted 295-amino acid human protein encoded by NAPA shares 37%, 60%, and 67% identity with the sequences of yeast, Drosophila, and squid alpha-SNAP, respectively. Platelets contain some of the same proteins, including NSF, p115/TAP, alpha-SNAP, gamma-SNAP, and the t-SNAREs syntaxin-2 and syntaxin-4, that are used in many vesicular transport processes in other cell types. Platelet exocytosis uses a molecular mechanism similar to that used by other secretory cells, such as neurons, although the proteins used by the platelet and their modes of regulation may be quite different. [provided by RefSeq]
• Synonyms: SNAPA
• Note: Seq homology of immunogen across species: Rhesus Monkey (100%), Chimpanzee (100%)