SDHB Rabbit Polyclonal Antibody

CAT#: TA308686

Rabbit Polyclonal antibody to SDHB (succinate dehydrogenase complex, subunit B, iron sulfur (Ip))

Product Images

SDHB antibody [C2C3], C-term detects SDHB protein by Western blot analysis. A. 30 ug THP-1 whole cell lysate/extract. B. 30 ug NCI-H929 whole cell lysate/extract. 12 % SDS-PAGE. SDHB antibody [C2C3], C-term (TA308686) dilution: 1:1000

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  SDHB antibody [C2C3], C-term detects SDHB protein by Western blot analysis. A. 30 ug Neuro2A whole cell lysate/extract. 12 % SDS-PAGE. SDHB antibody [C2C3], C-term (TA308686) dilution: 1:1000

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  Immunofluorescence analysis of methanol-fixed HeLa, using SDHB (TA308686) antibody at 1:200 dilution.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; WB:1:500-1:3000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to a region within amino acids 221 and 280 of SDHB (Uniprot ID#P21912)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 32 kDa
• Gene Name: succinate dehydrogenase complex iron sulfur subunit B
• Database Link:
  NP_002991
  Entrez Gene 298596 RatEntrez Gene 6390 Human
  UniProt ID P21912
• Background: Complex II of the respiratory chain, which is specifically involved in the oxidation of succinate, carries electrons from FADH to CoQ. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. The iron-sulfur subunit is highly conserved and contains three cysteine-rich clusters which may comprise the iron-sulfur centers of the enzyme. Sporadic and familial mutations in this gene result in paragangliomas and pheochromocytoma, and support a link between mitochondrial dysfunction and tumorigenesis. [provided by RefSeq]
• Synonyms: CWS2; IP; PGL4; SDH; SDH1; SDH2; SDHIP
• Note: Seq homology of immunogen across species: Rat (87%), Pig (100%), Rhesus Monkey (100%), Bovine (89%)

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Alzheimer's disease, Citrate cycle (TCA cycle), Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease