PDHA1 Rabbit Polyclonal Antibody

CAT#: TA308687

Rabbit Polyclonal antibody to Pyruvate Dehydrogenase E1 alpha (pyruvate dehydrogenase (lipoamide) alpha 1)

Product Images

Sample (20 ug of whole cell lysate). A: mouse brain. 10% SDS PAGE. TA308687 diluted at 1:5000.

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  Sample (50 ug of whole cell lysate). A: Rat brain. 10% SDS PAGE. TA308687 diluted at 1:3000.

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  Sample (30 ug of whole cell lysate). A:Hep G2. B:MOLT4. 12% SDS PAGE. TA308687 diluted at 1:1000

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  Pyruvate Dehydrogenase E1 alpha antibody detects PDHA1 protein at cytosol on hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. Pyruvate Dehydrogenase E1 alpha antibody (TA308687) dilution: 1:500.

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  Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Pyruvate Dehydrogenase E1 alpha (TA308687) antibody at 1:200 dilution.

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Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:1000-1:10000
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment corresponding to a region within amino acids 39 and 325 of (Uniprot ID#P08559)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 43 kDa
• Gene Name: pyruvate dehydrogenase (lipoamide) alpha 1
• Database Link:
  NP_000275
  Entrez Gene 18597 MouseEntrez Gene 5160 Human
  UniProt ID P08559
• Background: The pyruvate dehydrogenase complex is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA. The PDH complex is composed of multiple copies of 3 enzymes: E1 (PDHA1); dihydrolipoyl transacetylase (DLAT; MIM 608770) (E2; EC 2.3.1.12); and dihydrolipoyl dehydrogenase (DLD; MIM 238331) (E3; EC 1.8.1.4). The E1 enzyme is a heterotetramer of 2 alpha and 2 beta subunits. The E1-alpha subunit contains the E1 active site and plays a key role in the function of the PDH complex (Brown et al., 1994 [PubMed 7853374]). [supplied by OMIM]
• Synonyms: PDHA; PDHAD; PDHCE1A; PHE1A
• Note: Seq homology of immunogen across species: Rhesus Monkey (100%), Chimpanzee (100%)

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Butanoate metabolism, Citrate cycle (TCA cycle), Glycolysis / Gluconeogenesis, Metabolic pathways, Pyruvate metabolism, Valine, leucine and isoleucine biosynthesis