PAX8 Rabbit Polyclonal Antibody

CAT#: TA308698

Rabbit polyclonal antibody to PAX8CC (paired box 8)

Product Images

Sample (30 ug whole cell lysate). A:293T. 10% SDS PAGE. TA308698 diluted at 1:1000

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  PAX8 antibody detects PAX8 protein at nucleus on BT483 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded BT483 xenograft. PAX8 antibody (TA308698) dilution: 1:500.

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  Immunofluorescence analysis of paraformaldehyde-fixed A549, using PAX8 (TA308698) antibody at 1:200 dilution.

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  PAX8 antibody immunoprecipitates PAX8 protein in IP experiments. IP Sample: 1000 ug 293T whole cell lysate/extract A. 40 ug 293T whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of PAX8 protein by 2 ug of PAX8

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  PAX8 antibody immunoprecipitates PAX8 protein-DNA in ChIP experiments. ChIP Sample: 293T whole cell lysate/extract A. 5 ug preimmune rabbit IgG B. 5 ug of PAX8 antibody (TA308698) The precipitated DNA was detected by PCR with primer set targeting to E2F1

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Specifications

Product Data

• Applications: Assay, IF, IHC, IP, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:3000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 204 of PAX8
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 43 kDa
• Gene Name: paired box 8
• Database Link:
  NP_054698
  Entrez Gene 7849 Human
  UniProt ID Q06710
• Background: This gene encodes a member of the paired box (PAX) family of transcription factors. Members of this gene family typically encode proteins that contain a paired box domain, an octapeptide, and a paired-type homeodomain. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes. Mutations in this gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas and atypical follicular thyroid adenomas. Alternateively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq]
• Synonyms: OTTHUMP00000158659; OTTHUMP00000158660; paired box 8; paired domain gene 8

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Pathways in cancer, Thyroid cancer