Protein Kinase D2 (PRKD2) Rabbit Polyclonal Antibody

CAT#: TA308724

Rabbit Polyclonal antibody to PKD2 (protein kinase D2)

Product Images

Sample (30 ug of whole cell lysate). A: Molt-4. 7.5% SDS PAGE. TA308724 diluted at 1:1000.

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  PKD2 antibody [C1C3] detects PKD2 protein at cytosol and membrane on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. PKD2 antibody [C1C3] (TA308724) dilution: 1:500.

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  PKD2 antibody [C1C3] detects PKD2 protein at cytosol and membrane on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. PKD2 antibody [C1C3] (TA308724) dilution: 1:500.

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  PKD2 antibody [C1C3] detects PKD2 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: PKD2 protein stained by PKD2 antibody [C1C3] (TA308724) diluted at 1:200. Blue: Hoechst 33342 staining.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:3000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment corresponding to a region within amino acids 529 and 862 of PKD2 (Uniprot ID#Q9BZL6)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 97 kDa
• Gene Name: protein kinase D2
• Database Link:
  NP_001073351
  Entrez Gene 25865 Human
  UniProt ID Q9BZL6
• Background: The protein encoded by this gene belongs to the protein kinase D (PKD) family of serine/threonine protein kinases. This kinase can be activated by phorbol esters as well as by gastrin via the cholecystokinin B receptor (CCKBR) in gastric cancer cells. It can bind to diacylglycerol (DAG) in the trans-Golgi network (TGN) and may regulate basolateral membrane protein exit from TGN. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]
• Synonyms: HSPC187; nPKC-D2; PKD2
• Note: Seq homology of immunogen across species: Mouse (100%)

Reference Data

• Protein Families: Druggable Genome, Protein Kinase