Fatty Acid Synthase (FASN) Rabbit Polyclonal Antibody

CAT#: TA308750

Rabbit Polyclonal antibody to Fatty Acid Synthase (fatty acid synthase)

Product Images

Fatty Acid Synthase antibody [N1], N-term detects FASN protein by Western blot analysis. A. 30 ug PC-12 whole cell lysate/extract. B. 30 ug Rat2 whole cell lysate/extract. 5 % SDS-PAGE. Fatty Acid Synthase antibody [N1], N-term (TA308750) dilution: 1:1000

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  Sample (50 ug of whole cell lysate). A: Mouse brain. 5% SDS PAGE. TA308750 diluted at 1:1000.

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  Sample (30 ug of whole cell lysate). A: Molt-4. 7.5% SDS PAGE. TA308750 diluted at 1:1000

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  Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using Fatty Acid Synthase (TA308750) antibody at 1:500 dilution.

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  Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Fatty Acid Synthase (TA308750) antibody at 1:200 dilution.

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  FASN antibody immunoprecipitates FASN protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A : 30 ug whole cell lysate/extract of FASN protein expressing HeLa cells B : Control with 2.5 ug of pre-immune rabbit IgG C : Immunoprecipitation o

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Specifications

Product Data

• Applications: IF, IHC, IP, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:3000
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment corresponding to a region within amino acids 7 and 287 of Fatty Acid Synthase (Uniprot ID#P49327)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 273 kDa
• Gene Name: fatty acid synthase
• Database Link:
  NP_004095
  Entrez Gene 14104 MouseEntrez Gene 50671 RatEntrez Gene 2194 Human
  UniProt ID P49327
• Background: The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha. [provided by RefSeq]
• Synonyms: FAS; OA-519; SDR27X1
• Note: Seq homology of immunogen across species: Pig (83%), Rat (89%), Bovine (85%)

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Fatty acid biosynthesis, Insulin signaling pathway, Metabolic pathways