DNA Polymerase gamma (POLG) Rabbit Polyclonal Antibody

CAT#: TA308753

Rabbit Polyclonal antibody to DNA polymerase gamma (polymerase (DNA directed), gamma)

Product Images

Sample (30 ug of whole cell lysate). A: MCF-7. 5% SDS PAGE. TA308753 diluted at 1:1000.

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  DNA polymerase gamma antibody detects POLG protein by Western blot analysis. A. 50 ug Rat testis lysate/extract. 5% SDS-PAGE. DNA polymerase gamma antibody (TA308753) dilution: 1:1000.

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  Immunohistochemical analysis of paraffin-embedded Skeletal Muscle, using DNA polymerase gamma (TA308753) antibody (10 ug/ml).

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  Immunofluorescence analysis of methanol-fixed HeLa, using DNA polymerase gamma (TA308753) antibody at 1:200 dilution.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:3000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment corresponding to a region within amino acids 779 and 1231 of DNA polymerase gamma (Uniprot ID#P54098)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 140 kDa
• Gene Name: polymerase (DNA) gamma, catalytic subunit
• Database Link:
  NP_001119603
  Entrez Gene 5428 Human
  UniProt ID P54098
• Background: Mitochondrial DNA polymerase is heterotrimeric, consisting of a homodimer of accessory subunits plus a catalytic subunit. The protein encoded by this gene is the catalytic subunit of mitochondrial DNA polymerase. The encoded protein contains a polyglutamine tract near its N-terminus that may be polymorphic. Defects in this gene are a cause of progressive external ophthalmoplegia with mitochondrial DNA deletions 1 (PEOA1), sensory ataxic neuropathy dysarthria and ophthalmoparesis (SANDO), Alpers-Huttenlocher syndrome (AHS), and mitochondrial neurogastrointestinal encephalopathy syndrome (MNGIE). Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]
• Synonyms: MDP1; MIRAS; MTDPS4A; MTDPS4B; PEO; POLG1; POLGA; SANDO; SCAE

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways