RAD18 Rabbit Polyclonal Antibody

CAT#: TA308755

Rabbit polyclonal antibody to RAD18 (RAD18 homolog (S. cerevisiae))

Product Images

Sample (30 ug of whole cell lysate). A: Hep G2. 7.5% SDS PAGE. TA308755 diluted at 1:1000.

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  Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft, using RAD18 (TA308755) antibody at 1:100 dilution.

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  RAD18 antibody [N1C1] detects RAD18 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RAD18 protein stained by RAD18 antibody [N1C1] (TA308755) diluted at 1:500. Blue: Hoechst 3

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:3000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 259 of RAD18 (Uniprot ID#Q9NS91)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 56 kDa
• Gene Name: RAD18, E3 ubiquitin protein ligase
• Database Link:
  NP_064550
  Entrez Gene 56852 Human
  UniProt ID Q9NS91
• Background: The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens. [provided by RefSeq]
• Synonyms: RNF73
• Note: Seq homology of immunogen across species: Bovine (80%)

Reference Data

• Protein Families: Druggable Genome